ADVANCED OPTICAL MICROSCOPY FOR LIFE AND MATERIAL SCIENCE
cod. 1006877

Anno accademico 2016/17
1° anno di corso - Secondo semestre
Docente
Settore scientifico disciplinare
Fisica applicata (a beni culturali, ambientali, biologia e medicina) (FIS/07)
Field
A scelta dello studente
Tipologia attività formativa
A scelta dello studente
52 ore
di attività frontali
6 crediti
sede: PARMA
insegnamento
in - - -

Obiettivi formativi

Offer an overview of the principles of modern light miscroscopies in life and materials science.

Prerequisiti

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Contenuti dell'insegnamento

Theory of light, light matter interaction, with an emphasis on fluorescence
The Light Microscope: history and evolution, components of a compound microscope
Light microscopy
- The optical components of compound microscope and their functions/operations
- The physics of light and glass
- Forming and maintaining optical resolution with these lenses
- Proper(Kohler) bright field illumination
- Principles of sample preparation for light microscopy
- Theory of image formation, diffraction-limited resolution
- Optical aberrations and their correction
- Illumination and image forming techniques:
* Brightfield Optics
* Oblique Illumination Techniques
* Imaging of Phase Objects
* Polarization Optics
* Fluorescence Microscopy
* Probes for fluorescence microscopy
* Confocal Microscopy
* Single molecule microscopy and applications in biology
* FCS and applications in biology
- Labeling and labeling strategies
- DNA sequencing with emphasis on single molecule optical approaches
- Non-linear microscopy: Multi-photon excitation, Second Harmonic Imaging, different Raman microscopy schemes
- New microscopies to improve resolution
- STED, structured light microscopy, PALM/STORM
Case studies in biology, biotechnology, material science,.

• Structural and functional analysis of cellular and subcellular entities at various length scales and with sensitivities down to the single molecule level via optical microscopy
• Introduction to DNA sequencing, an overview of the different technologies. The role of microscopy in DNA sequencing will be explained. Optical mapping as a coarse (compared to sequencing) but useful DNA tool will be outlined.
• Technical principles of electron microscopy (EM) and atomic force microscopy (AFM) will be explained. Salient examples of the use of EM and AFM in a nano-biological context will be explained. The link with optical microscopy and the differences in resolution, dynamics, contrast, will be emphasized.

Programma esteso

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Bibliografia

Original research papers and reviews published on scientific journals

Metodi didattici

The course consists in a series of lectures covering the fundamentals of advanced light microscopies.
Part of the course will be the reading of a microscopy related paper and presentation discussion on the paper in one of the classes.
A selection of papers will be presented from which students can choose for their presentation.

Modalità verifica apprendimento

Oral. 25 % of the points will be given for the presentation on a paper chosen from a selection.

Altre informazioni

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